PUZZLE 4.0.2 Type of analysis: tree reconstruction Parameter estimation: approximate (faster) Parameter estimation uses: neighbor-joining tree (for substitution process and rate variation) Standard errors (S.E.) are obtained by the curvature method. The upper and lower bounds of an approximate 95% confidence interval for parameter or branch length x are x-1.96*S.E. and x+1.96*S.E. SEQUENCE ALIGNMENT Input data: 33 sequences with 669 amino acid sites Number of constant sites: 0 (= 0.0% of all sites) SUBSTITUTION PROCESS Model of substitution: JTT (Jones et al. 1992) Amino acid frequencies (estimated from data set): pi(A) = 9.3% pi(R) = 5.8% pi(N) = 2.6% pi(D) = 4.7% pi(C) = 0.7% pi(Q) = 2.7% pi(E) = 6.8% pi(G) = 7.5% pi(H) = 1.9% pi(I) = 8.1% pi(L) = 12.4% pi(K) = 4.5% pi(M) = 2.7% pi(F) = 4.4% pi(P) = 3.4% pi(S) = 5.7% pi(T) = 5.1% pi(W) = 0.8% pi(Y) = 2.3% pi(V) = 8.6% RATE HETEROGENEITY Model of rate heterogeneity: uniform rate SEQUENCES IN INPUT ORDER 5% chi-square test p-value Methanococ failed 0.00% [590] Methanoco2 failed 0.00% [594] Ecoli failed 4.54% [591] *Klebsiell failed 0.05% [540] *Ecoli9 failed 0.00% [666] *Myxococcu failed 0.00% [601] Ecoli7 failed 0.00% [846] Helicobact failed 0.53% [640] Helicobac2 failed 0.20% [658] *Streptomy failed 3.29% [710] *Mycobacte failed 1.43% [866] *Azorhizob failed 1.37% [912] Vibrio passed 5.25% [802] *Haemophil passed 93.13% [655] *Arabidops failed 0.11% [704] Archaeoglo passed 5.79% [664] Deinococcu failed 0.20% [718] Synechocys passed 12.25% [566] Thermotoga passed 34.55% [624] Synechocy2 failed 1.30% [664] *Methanoba passed 5.14% [679] *Pyrococcu failed 0.12% [841] *Aquifex2 failed 3.18% [722] *Ecoli8 failed 0.12% [832] Methanoba3 passed 26.67% [689] *Synechocy passed 53.39% [758] *Mycobact2 failed 0.61% [798] Mycobacter failed 0.02% [632] Methanobac failed 0.00% [1285] *Synechoc2 failed 0.65% [1903] Aquifex failed 0.00% [1643] *Bacillus passed 24.52% [1894] *Archaeogl failed 1.56% [1793] The chi-square tests compares the amino acid composition of each sequence to the frequency distribution assumed in the maximum likelihood model. Warning: Result of chi-square test may not be valid because of small maximum likelihood frequencies and short sequence length! The number in square brackets indicates how often each sequence is involved in one of the 7020 completely unresolved quartets of the quartet puzzling tree search. IDENTICAL SEQUENCES The sequences in each of the following groups are all identical. To speed up computation please remove all but one of each group from the data set. All sequences are unique. MAXIMUM LIKELIHOOD DISTANCES Maximum likelihood distances are computed using the selected model of substitution and rate heterogeneity. 33 Methanococ 0.00000 0.87354 8.15511 7.69351 7.91237 8.99866 7.30913 2.69990 2.69657 8.99882 8.90572 8.99826 7.72581 8.99827 8.99842 1.75577 2.06171 1.82467 1.78015 1.94865 8.99820 8.81137 2.51254 8.99856 2.30972 8.99866 6.34384 3.21408 6.29256 7.25024 8.06201 8.02068 6.15479 Methanoco2 0.87354 0.00000 7.35985 7.91689 7.86888 8.99903 6.36513 2.78415 2.75540 8.99861 8.98293 8.99832 8.99839 7.11414 8.99823 1.82336 1.88294 1.98265 1.80013 2.10421 8.03209 8.99849 2.45631 8.99842 2.69419 7.75353 7.98267 3.10245 6.40685 8.09854 8.53464 8.99836 7.02837 Ecoli 8.15511 7.35985 0.00000 0.21603 1.07678 1.17988 4.01471 7.70145 7.49981 8.99865 8.99889 8.99833 8.14163 1.37946 1.59670 8.99822 8.99849 8.99849 8.59534 8.99892 2.15774 8.99901 8.99820 2.23231 8.99838 6.03953 8.99900 8.99854 8.99904 8.99873 6.75914 6.99013 8.56697 *Klebsiell 7.69351 7.91689 0.21603 0.00000 1.10091 1.25168 4.01743 7.36962 7.17104 8.99824 8.99906 8.99884 8.02137 1.47104 1.65675 8.99835 8.99892 8.82879 8.07228 8.99854 2.11977 8.99894 8.99844 2.17042 8.99821 7.00867 8.99851 8.99859 8.99897 8.99872 6.84036 6.01482 8.88478 *Ecoli9 7.91237 7.86888 1.07678 1.10091 0.00000 1.05007 4.00082 8.99841 8.99835 8.99909 8.99861 8.99907 8.95927 1.41514 1.58820 8.99838 7.51768 8.22167 8.99844 8.99850 2.21400 8.99843 8.99831 2.12631 8.99821 8.99908 8.99897 8.99842 8.99889 8.99862 6.95922 8.72915 8.99830 *Myxococcu 8.99866 8.99903 1.17988 1.25168 1.05007 0.00000 4.39270 6.88420 6.90629 8.99838 8.99833 8.99897 8.99839 1.57963 1.53948 8.11904 8.99908 7.53955 8.99888 8.99846 2.12396 8.99889 8.99866 2.27771 8.99842 7.88243 8.99828 8.99830 8.99873 8.99892 6.16496 7.97738 8.99904 Ecoli7 7.30913 6.36513 4.01471 4.01743 4.00082 4.39270 0.00000 8.07962 7.92296 8.99865 8.99843 8.99896 7.80843 4.40019 5.35921 8.06874 8.99903 6.12628 7.15822 8.55280 5.17386 8.99843 7.24418 6.73338 8.99851 8.99888 8.99889 7.77503 7.91797 8.99884 6.18723 8.40098 8.99823 Helicobact 2.69990 2.78415 7.70145 7.36962 8.99841 6.88420 8.07962 0.00000 0.01869 8.99848 8.95794 8.99832 8.14889 7.87669 8.99839 3.18525 2.89536 2.75843 2.92248 3.31266 8.47802 8.99823 2.61899 8.99851 3.63683 8.99895 8.99855 5.40296 7.34041 8.77107 7.01923 8.99843 8.99844 Helicobac2 2.69657 2.75540 7.49981 7.17104 8.99835 6.90629 7.92296 0.01869 0.00000 8.95645 8.69757 8.99831 8.04185 7.87031 8.99858 3.16862 2.94290 2.73661 2.86804 3.31172 8.18052 8.99895 2.58676 8.99835 3.64626 8.99897 8.99893 5.51554 7.38308 8.73821 6.89059 8.99838 8.99908 *Streptomy 8.99882 8.99861 8.99865 8.99824 8.99909 8.99838 8.99865 8.99848 8.95645 0.00000 0.66891 1.79970 1.69216 8.99834 7.66176 8.99834 8.99854 8.99873 8.99839 8.99898 8.99839 2.21846 8.99904 8.05091 8.99830 2.91089 2.58355 8.99834 4.20898 8.99877 8.99840 8.99852 6.32061 *Mycobacte 8.90572 8.98293 8.99889 8.99906 8.99861 8.99833 8.99843 8.95794 8.69757 0.66891 0.00000 1.75247 2.26758 8.99876 7.06582 8.99854 8.99852 8.99905 8.99863 7.43556 6.35193 2.48783 8.99841 8.99847 8.99874 3.02824 2.28390 8.99823 6.03562 8.99878 8.99834 8.99896 7.03961 *Azorhizob 8.99826 8.99832 8.99833 8.99884 8.99907 8.99897 8.99896 8.99832 8.99831 1.79970 1.75247 0.00000 0.92707 8.99837 8.99881 8.99893 8.99884 8.99841 8.99823 8.99835 8.99821 2.05079 8.99904 8.99890 8.99838 2.53027 1.99636 6.00296 5.07945 8.99837 8.99835 8.47421 8.99908 Vibrio 7.72581 8.99839 8.14163 8.02137 8.95927 8.99839 7.80843 8.14889 8.04185 1.69216 2.26758 0.92707 0.00000 8.11011 7.42690 8.99856 8.99842 6.98528 8.99860 8.99876 8.99875 2.40633 7.55082 8.99897 7.55179 3.28879 2.61411 8.87185 6.52466 8.99848 8.99867 8.99824 8.07178 *Haemophil 8.99827 7.11414 1.37946 1.47104 1.41514 1.57963 4.40019 7.87669 7.87031 8.99834 8.99876 8.99837 8.11011 0.00000 1.60231 8.99840 8.99832 8.99823 8.99843 8.99845 2.07092 8.99856 8.99840 2.10516 8.99840 6.20956 8.38560 8.99904 8.99883 8.99894 7.05788 8.99830 8.36994 *Arabidops 8.99842 8.99823 1.59670 1.65675 1.58820 1.53948 5.35921 8.99839 8.99858 7.66176 7.06582 8.99881 7.42690 1.60231 0.00000 8.99883 8.99835 8.99887 8.99851 8.99847 1.92501 8.99890 8.99864 2.13543 8.99841 5.16662 8.82722 8.99821 8.99888 8.99900 8.00922 8.99868 8.99848 Archaeoglo 1.75577 1.82336 8.99822 8.99835 8.99838 8.11904 8.06874 3.18525 3.16862 8.99834 8.99854 8.99893 8.99856 8.99840 8.99883 0.00000 1.99442 1.97147 2.24250 1.88213 8.99879 6.87228 3.16477 8.99853 2.88105 8.99894 7.58261 2.90292 6.14459 7.82641 7.49739 8.99821 8.99826 Deinococcu 2.06171 1.88294 8.99849 8.99892 7.51768 8.99908 8.99903 2.89536 2.94290 8.99854 8.99852 8.99884 8.99842 8.99832 8.99835 1.99442 0.00000 1.44191 2.00121 2.35074 8.53127 8.99842 2.99666 8.99905 2.42057 8.94861 8.90971 2.82366 8.99837 5.89764 8.99875 8.99848 8.86134 Synechocys 1.82467 1.98265 8.99849 8.82879 8.22167 7.53955 6.12628 2.75843 2.73661 8.99873 8.99905 8.99841 6.98528 8.99823 8.99887 1.97147 1.44191 0.00000 1.60525 2.28125 8.99821 8.99860 2.35699 8.99851 2.39452 8.99825 8.99829 3.15222 6.76477 8.99849 8.78908 7.76684 6.23140 Thermotoga 1.78015 1.80013 8.59534 8.07228 8.99844 8.99888 7.15822 2.92248 2.86804 8.99839 8.99863 8.99823 8.99860 8.99843 8.99851 2.24250 2.00121 1.60525 0.00000 2.30786 7.90792 8.99859 2.28102 8.99880 2.49748 8.99890 8.02934 3.02961 8.99400 7.93889 7.03417 8.99855 7.43553 Synechocy2 1.94865 2.10421 8.99892 8.99854 8.99850 8.99846 8.55280 3.31266 3.31172 8.99898 7.43556 8.99835 8.99876 8.99845 8.99847 1.88213 2.35074 2.28125 2.30786 0.00000 8.99868 8.99887 3.04994 8.99858 3.07137 8.99894 7.26588 3.54121 6.28482 7.10738 8.99901 8.99873 6.21249 *Methanoba 8.99820 8.03209 2.15774 2.11977 2.21400 2.12396 5.17386 8.47802 8.18052 8.99839 6.35193 8.99821 8.99875 2.07092 1.92501 8.99879 8.53127 8.99821 7.90792 8.99868 0.00000 8.17988 8.99831 2.22644 7.39262 7.23428 6.65926 7.51890 8.99898 8.99897 6.85417 8.99906 7.74612 *Pyrococcu 8.81137 8.99849 8.99901 8.99894 8.99843 8.99889 8.99843 8.99823 8.99895 2.21846 2.48783 2.05079 2.40633 8.99856 8.99890 6.87228 8.99842 8.99860 8.99859 8.99887 8.17988 0.00000 8.99832 8.99873 8.99831 3.35019 2.58175 8.99829 5.10683 8.99907 7.94303 8.99886 8.28602 *Aquifex2 2.51254 2.45631 8.99820 8.99844 8.99831 8.99866 7.24418 2.61899 2.58676 8.99904 8.99841 8.99904 7.55082 8.99840 8.99864 3.16477 2.99666 2.35699 2.28102 3.04994 8.99831 8.99832 0.00000 8.99855 2.82270 8.99821 8.99894 4.40142 7.33619 8.92970 8.17168 8.99862 8.99828 *Ecoli8 8.99856 8.99842 2.23231 2.17042 2.12631 2.27771 6.73338 8.99851 8.99835 8.05091 8.99847 8.99890 8.99897 2.10516 2.13543 8.99853 8.99905 8.99851 8.99880 8.99858 2.22644 8.99873 8.99855 0.00000 8.99844 6.92313 8.99825 8.41112 8.99833 8.99859 6.75053 8.99845 8.99869 Methanoba3 2.30972 2.69419 8.99838 8.99821 8.99821 8.99842 8.99851 3.63683 3.64626 8.99830 8.99874 8.99838 7.55179 8.99840 8.99841 2.88105 2.42057 2.39452 2.49748 3.07137 7.39262 8.99831 2.82270 8.99844 0.00000 8.99835 5.38037 3.45812 8.17126 8.99845 8.08850 8.49738 8.99850 *Synechocy 8.99866 7.75353 6.03953 7.00867 8.99908 7.88243 8.99888 8.99895 8.99897 2.91089 3.02824 2.53027 3.28879 6.20956 5.16662 8.99894 8.94861 8.99825 8.99890 8.99894 7.23428 3.35019 8.99821 6.92313 8.99835 0.00000 3.69403 6.63449 5.40227 8.99872 8.99835 8.99845 7.81377 *Mycobact2 6.34384 7.98267 8.99900 8.99851 8.99897 8.99828 8.99889 8.99855 8.99893 2.58355 2.28390 1.99636 2.61411 8.38560 8.82722 7.58261 8.90971 8.99829 8.02934 7.26588 6.65926 2.58175 8.99894 8.99825 5.38037 3.69403 0.00000 6.29576 5.38366 7.63488 7.57062 8.99890 7.07644 Mycobacter 3.21408 3.10245 8.99854 8.99859 8.99842 8.99830 7.77503 5.40296 5.51554 8.99834 8.99823 6.00296 8.87185 8.99904 8.99821 2.90292 2.82366 3.15222 3.02961 3.54121 7.51890 8.99829 4.40142 8.41112 3.45812 6.63449 6.29576 0.00000 6.22742 7.45901 8.99883 8.99837 7.13380 Methanobac 6.29256 6.40685 8.99904 8.99897 8.99889 8.99873 7.91797 7.34041 7.38308 4.20898 6.03562 5.07945 6.52466 8.99883 8.99888 6.14459 8.99837 6.76477 8.99400 6.28482 8.99898 5.10683 7.33619 8.99833 8.17126 5.40227 5.38366 6.22742 0.00000 8.99879 8.99849 8.99889 6.76027 *Synechoc2 7.25024 8.09854 8.99873 8.99872 8.99862 8.99892 8.99884 8.77107 8.73821 8.99877 8.99878 8.99837 8.99848 8.99894 8.99900 7.82641 5.89764 8.99849 7.93889 7.10738 8.99897 8.99907 8.92970 8.99859 8.99845 8.99872 7.63488 7.45901 8.99879 0.00000 8.99899 8.65691 8.99878 Aquifex 8.06201 8.53464 6.75914 6.84036 6.95922 6.16496 6.18723 7.01923 6.89059 8.99840 8.99834 8.99835 8.99867 7.05788 8.00922 7.49739 8.99875 8.78908 7.03417 8.99901 6.85417 7.94303 8.17168 6.75053 8.08850 8.99835 7.57062 8.99883 8.99849 8.99899 0.00000 8.99862 8.99828 *Bacillus 8.02068 8.99836 6.99013 6.01482 8.72915 7.97738 8.40098 8.99843 8.99838 8.99852 8.99896 8.47421 8.99824 8.99830 8.99868 8.99821 8.99848 7.76684 8.99855 8.99873 8.99906 8.99886 8.99862 8.99845 8.49738 8.99845 8.99890 8.99837 8.99889 8.65691 8.99862 0.00000 8.92342 *Archaeogl 6.15479 7.02837 8.56697 8.88478 8.99830 8.99904 8.99823 8.99844 8.99908 6.32061 7.03961 8.99908 8.07178 8.36994 8.99848 8.99826 8.86134 6.23140 7.43553 6.21249 7.74612 8.28602 8.99828 8.99869 8.99850 7.81377 7.07644 7.13380 6.76027 8.99878 8.99828 8.92342 0.00000 Average distance (over all possible pairs of sequences): 7.01453 TREE SEARCH Quartet puzzling is used to choose from the possible tree topologies and to simultaneously infer support values for internal branches. Number of puzzling steps: 10000 Analysed quartets: 40920 Unresolved quartets: 7020 (= 17.2%) Quartet trees are based on approximate maximum likelihood values using the selected model of substitution and rate heterogeneity. QUARTET PUZZLING TREE Support for the internal branches of the unrooted quartet puzzling tree topology is shown in percent. This quartet puzzling tree is not completely resolved! :---*Azorhizob :---------84: : :---Vibrio : : :---Ecoli : :-81: : : :---*Klebsiell : : : : :---*Methanoba : :-81: : : :---*Ecoli8 : :-53: : : : :---*Haemophil : : :-78: : : : :---*Arabidops :-56: : : : : :---*Ecoli9 : : :-65: : : :---*Myxococcu : : :-93: :-----------Ecoli7 : : : :---------------*Streptomy : : : :---------------*Mycobacte : : : :---------------*Pyrococcu : : : :---------------*Synechocy : : : :---------------*Mycobact2 : : : :---------------Methanobac : : : :---------------*Synechoc2 : : : :---------------Aquifex : : : :---------------*Bacillus : : :-84: :---------------*Archaeogl : : : : :---Archaeoglo : :-------------78: : : :---Synechocy2 : : : : :---Helicobact : : :-84: : :---------56: :---Helicobac2 : : : : : :-------*Aquifex2 : : : :-------------------Deinococcu : : : :-------------------Synechocys : : : :-------------------Thermotoga : : : :-------------------Methanoba3 : : : :-------------------Mycobacter : :-----------------------Methanoco2 : :-----------------------Methanococ Quartet puzzling tree (in CLUSTAL W notation): (Methanococ,(((*Azorhizob,Vibrio)84,(((Ecoli,*Klebsiell)81, (*Methanoba,*Ecoli8)81,(*Haemophil,*Arabidops)78,(*Ecoli9, *Myxococcu)65)53,Ecoli7)56,*Streptomy,*Mycobacte,*Pyrococcu, *Synechocy,*Mycobact2,Methanobac,*Synechoc2,Aquifex,*Bacillus, *Archaeogl)93,(Archaeoglo,Synechocy2)78,((Helicobact,Helicobac2)84, *Aquifex2)56,Deinococcu,Synechocys,Thermotoga,Methanoba3,Mycobacter)84, Methanoco2); BIPARTITIONS The following bipartitions occured at least once in all intermediate trees that have been generated in the 10000 puzzling steps: Bipartitions included in the quartet puzzling tree: (bipartition with sequences in input order : number of times seen) **.....**. .....***** ..*.*..*.. ... : 9312 *******..* ********** ********** *** : 8441 ********** *..******* ********** *** : 8429 **........ .......... .......... ... : 8403 **..****** ********** ********** *** : 8076 ********** ********** .**.****** *** : 8054 ********** ***..***** ********** *** : 7772 ********** *****.***. ********** *** : 7759 ****..**** ********** ********** *** : 6467 *******..* ********** **.******* *** : 5553 **.....*** ***..***** .**.****** *** : 5551 **....**** ***..***** .**.****** *** : 5265 Bipartitions not included in the quartet puzzling tree: (bipartition with sequences in input order : number of times seen) **....**** ********** ********** *** : 4948 **....**** ***..***** ********** *** : 4938 *********. ...******* *.***..*.* *** : 4937 **........ .....*...* .......... ... : 4901 *********. .********* ********** *** : 4789 ********** ******..** ********** *** : 4180 *********. ...******* *.***..*** *** : 3596 ********** ********** *********. *.* : 3554 ********** *******..* ********** *** : 3028 **.....**. .....***** ..*.*..... ... : 3019 ********** ********** *.****.*** *** : 2818 **.....*** ***..***** ********** *** : 2815 **.....*** ***..***** .**.****** .** : 2802 ********** ******...* ********** *** : 2722 ********** ********** *********. *.. : 2652 ******.*** ********** ********** .** : 2564 ********** ********** *********. **. : 2528 ********** ******.*** *******.** *** : 2374 **.....*** ********** ********** *** : 2354 ********** ********** ********.* **. : 2340 (2663 other less frequent bipartitions not shown) MAXIMUM LIKELIHOOD BRANCH LENGTHS ON QUARTET PUZZLING TREE (NO CLOCK) Branch lengths are computed using the selected model of substitution and rate heterogeneity. :--12 *Azorhizob :----34 : :--13 Vibrio : : :-3 Ecoli : :--35 : : :-4 *Klebsiell : : : : :----21 *Methanoba : :--36 : : :----24 *Ecoli8 : :-----------39 : : : :---14 *Haemophil : : :-37 : : : :---15 *Arabidops : : : : : : :--5 *Ecoli9 : : :-38 : : :--6 *Myxococcu :--------------------40 : :-7 Ecoli7 : :-10 *Streptomy : :--11 *Mycobacte : :------22 *Pyrococcu : :--------26 *Synechocy :---------------------41 : :------27 *Mycobact2 : : : :--------------29 Methanobac : : : :---------------------------30 *Synechoc2 : : : :---------------------------31 Aquifex : : : :---------------------------32 *Bacillus : : : :-------------------33 *Archaeogl : : :---16 Archaeoglo :--42 : :----20 Synechocy2 : : :-8 Helicobact : :-----43 : : :-9 Helicobac2 :--44 : :----23 *Aquifex2 : :----17 Deinococcu :--45 : :---18 Synechocys : : : :---19 Thermotoga : : : :-----25 Methanoba3 : : : :--------28 Mycobacter : :--2 Methanoco2 : :--1 Methanococ branch length S.E. branch length S.E. Methanococ 1 0.44960 0.06025 34 1.11888 0.15633 Methanoco2 2 0.41684 0.05954 35 0.46862 0.04605 Ecoli 3 0.08631 0.01723 36 0.52201 0.07902 *Klebsiell 4 0.13106 0.01895 37 0.24716 0.04579 *Ecoli9 5 0.46894 0.04563 38 0.17732 0.03656 *Myxococcu 6 0.57837 0.05057 39 3.62217 0.36641 Ecoli7 7 0.00001 0.00023 40 6.55695 1.39499 Helicobact 8 0.01868 0.00708 41 6.93255 1.78757 Helicobac2 9 0.00001 0.00015 42 0.57071 0.09958 *Streptomy 10 0.22716 0.06135 43 1.41303 0.14743 *Mycobacte 11 0.52017 0.07719 44 0.52353 0.10238 *Azorhizob 12 0.57510 0.12079 45 0.60889 0.08080 Vibrio 13 0.41433 0.10654 *Haemophil 14 0.72079 0.06129 *Arabidops 15 0.85559 0.06866 Archaeoglo 16 0.83442 0.10705 Deinococcu 17 1.09619 0.12303 Synechocys 18 0.85679 0.08858 Thermotoga 19 0.93561 0.09217 Synechocy2 20 1.06963 0.12061 *Methanoba 21 1.06956 0.09924 *Pyrococcu 22 1.89294 0.21417 *Aquifex2 23 1.04787 0.12318 *Ecoli8 24 1.16147 0.10202 Methanoba3 25 1.55114 0.14408 *Synechocy 26 2.52504 0.30964 *Mycobact2 27 2.02112 0.22806 Mycobacter 28 2.50335 0.23819 Methanobac 29 4.51730 0.56698 *Synechoc2 30 8.99836 2.86802 Aquifex 31 8.99910 2.34920 *Bacillus 32 8.99879 2.21632 87 iterations until convergence *Archaeogl 33 6.08382 1.09022 log L: -31526.84 WARNING --- at least one brach length is close to an internal boundary! Quartet puzzling tree with maximum likelihood branch lengths (in CLUSTAL W notation): (Methanococ:0.44960,(((*Azorhizob:0.57510,Vibrio:0.41433)84:1.11888, (((Ecoli:0.08631,*Klebsiell:0.13106)81:0.46862,(*Methanoba:1.06956, *Ecoli8:1.16147)81:0.52201,(*Haemophil:0.72079,*Arabidops:0.85559) 78:0.24716,(*Ecoli9:0.46894,*Myxococcu:0.57837)65:0.17732)53:3.62217, Ecoli7:0.00001)56:6.55695,*Streptomy:0.22716,*Mycobacte:0.52017, *Pyrococcu:1.89294,*Synechocy:2.52504,*Mycobact2:2.02112,Methanobac:4.51730, *Synechoc2:8.99836,Aquifex:8.99910,*Bacillus:8.99879,*Archaeogl:6.08382) 93:6.93255,(Archaeoglo:0.83442,Synechocy2:1.06963)78:0.57071,((Helicobact:0.01868, Helicobac2:0.00001)84:1.41303,*Aquifex2:1.04787)56:0.52353,Deinococcu:1.09619, Synechocys:0.85679,Thermotoga:0.93561,Methanoba3:1.55114,Mycobacter:2.50335) 84:0.60889,Methanoco2:0.41684); TIME STAMP Date and time: Fri Dec 3 09:37:31 1999 Runtime: 9848 seconds (= 164.1 minutes = 2.7 hours)